Total Protein Assay Kits
Invent Biotechnologies Inc ALBUMIN DEPLETION REAGENT 20ML
Human/animal serum and plasma are the best sources of samples for biomarker identification. However, the presence of albumin in large amounts interferes with many assays and functional proteomic analysis. Serum albumin accounts for 50-70% of the total serum protein that causes loss of resolution for 1D and 2D electrophoresis. Effective removal of albumin using our reagent can increase sensitivity of the assay for many folds. Unlike a resin-based depletion kit (that only deals with microliter amounts of a sample), Minute albumin depletion reagent can handle a few microliters and up to several milliliters of samples. Over 90% of albumin can be removed in less than 5 minutes.
Medchemexpress LLC C-X-C motif chemokine 13 (CXCL13), cynomolgus, His-tag | >95.0% | 500 UG
Recombinant C-X-C motif chemokine 13 (CXCL13, also BCA-1) from cynomolgus, supplied as a His-tagged, lyophilized protein for research use. The protein is expressed in E. coli, provided with low endotoxin, and validated for chemoattractant activity in cell assays.
- His-tagged recombinant protein from cynomolgus
- Expressed in E. coli and lyophilized for stability
- Purity greater than 95% by reducing SDS-PAGE
- Endotoxin level <1 EU/μg (LAL method)
- Apparent molecular weight ~13 kDa; ED50 0.019 μg/mL in chemoattraction assay
- Formulated in Tris/NaCl; recommend aliquoting with carrier protein for long-term storage at -20°C
- Suitable for cell migration, receptor binding, and signaling studies
BioVendor, LLC QuickZyme Soluble Collagen Assay Kit 96-Assays
QuickZyme Soluble Collagen Assay Kit, 2 X 96-Assays. Measure all types of fibrillary collagen in conditioned cell culture media and cellular extracts. Fast & simple 96-well plate format! Species independent. Not affected by serum. Dynamic range: 0.5-10 ug/well. Sensitivity: 0.69 ug/well. Sample vol. 10 -140 ul. Assay time < 3 hrs. Collagen is one of the main components of extracellular matrix. Dysregulated collagen production results in numerous pathologies, thus collagen measurement is important in many disease related studies. The QuickZyme collagen assay is based on binding of Sirius Red dye to collagen. Following dye-binding, the collagen-dye complex precipitates, resulting in a colored pellet. This color can be released in an alkaline solution. After heating at 50C the signal is lost, as the assay does not recognize denatured collagen (gelatin). The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin.
BioVendor, LLC QuickZyme Soluble Collagen Assay Kit 96-Assays
QuickZyme Soluble Collagen Assay Kit, 96-Assays. Measure all types of fibrillary collagen in conditioned cell culture media and cellular extracts. Fast & simple 96-well plate format! Species independent. Not affected by serum. Dynamic range: 0.5-10 ug/well. Sensitivity: 0.69 ug/well. Sample vol. 10 -140 ul. Assay time < 3 hrs. Collagen is one of the main components of extracellular matrix. Dysregulated collagen production results in numerous pathologies, thus measurement of collagen is important in many disease related studies. The QuickZyme collagen assay is based on binding of Sirius Red dye to collagen. Following dye-binding, the collagen-dye complex precipitates, resulting in a colored pellet. This color can be released in an alkaline solution. After heating at 50C the signal is lost, as the assay does not recognize denatured collagen (gelatin). The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin.
Meso Scale Discovery U-PLEX Mouse BCA-1/BLC ASYQP
The Meso Scale Discovery U-PLEX Mouse BCA-1/BLC Assay is a multiplex immunoassay kit for measuring BCA-1 and BLC in mouse samples like serum plasma and cell culture supernatants It uses MSD s MULTI-ARRAY technology offering high sensitivity a wide dynamic range and low sample volume needs The kit includes a 96-well plate with capture and detection antibodies plus calibrators and controls for standard curve generation and assay validation This assay efficiently analyzes BCA-1 and BLC biomarkers crucial for immune function and inflammation
Medchemexpress LLC BCA-1/CXCL13 protein, cynomolgus (His tag) | 50 UG
Recombinant cynomolgus CXCL13 (BCA-1) is a chemokine that selectively attracts B lymphocytes. Supplied as a lyophilized 50 μg vial with an N-terminal His tag, the protein is expressed in E. coli and characterized for activity and purity for research use.
- Recombinant protein, cynomolgus origin.
- N-terminal His tag for purification and detection.
- Lyophilized formulation from 10 mM Tris, 250 mM NaCl, pH 8.0.
- Purity >95% as determined by reducing SDS-PAGE.
- Predicted molecular mass 10.3 kDa; apparent ~13 kDa by SDS-PAGE.
- Bioactivity: ED50 0.019 μg/mL in Ramos cell chemotaxis assay.
- Store lyophilized at -20°C; aliquot and store at -20°C or -80°C after reconstitution.
Medchemexpress LLC BCA-1/CXCL13 protein, cynomolgus (His tag) | >95.0% | 10 UG
Recombinant cynomolgus CXCL13 (BCA-1) is a His-tagged chemokine produced in E. coli for use in cell-based and biochemical assays. It acts as a B-lymphocyte chemoattractant and is supplied lyophilized with recommended reconstitution and storage instructions. Purity and bioactivity are validated by SDS-PAGE and cell migration assays.
- Recombinant cynomolgus CXCL13 (BCA-1), His-tagged.
- Expressed in E. coli.
- Purity >95.0% by reducing SDS-PAGE.
- Approximate molecular weight 13 kDa under reducing conditions.
- Lyophilized from 10 mM Tris, 250 mM NaCl, pH 8.0.
- Validated chemoattractant activity on Ramos cells (ED50 0.019 μg/mL).
- Store lyophilized at -20°C; aliquot and freeze at -20°C or -80°C after reconstitution.
Medchemexpress LLC BCA-1/CXCL13 Human 100ug
BCA-1/CXCL13 Protein, Human is a recombinant, tag-free chemokine produced in Escherichia coli and supplied as a lyophilized material for in vitro research. CXCL13 selectively attracts B lymphocytes via the CXCR5 receptor and has validated biological activity in chemotaxis and cell proliferation assays, making it suitable for functional and signaling studies.
- Recombinant human CXCL13 produced in Escherichia coli.
- Tag free, lyophilized preparation for stable storage.
- Purity greater than 95% as determined by reducing SDS-PAGE.
- Approximate molecular weight ~11 kDa under reducing conditions.
- Demonstrated chemotactic activity for human B cells; ED50 typically ~4.6 ng/mL.
- Supplied as a 100 μg vial suitable for in vitro assays.
- Store at -20°C; aliquot and freeze at -80°C for extended storage.
American Research Products Inc Anti CXCL13 BCA 1 BLC VX5 5261
Anti-Human CXCL13/BCA-1/BLC (VX5/5261) Research Grade
Medchemexpress LLC BCA-1/CXCL13 protein, rat (His-GST) | >90.0% | 20UG
Recombinant rat BCA-1 (CXCL13) is a CXC chemokine produced with an N-terminal His-GST tag in Escherichia coli and supplied lyophilized for research use. The protein is suitable for receptor binding studies, chemotaxis assays, and antibody generation, and is provided with characterized purity, molecular weight, and storage recommendations.
- Species: rat
- Tag: N-terminal His-GST
- Expression system: Escherichia coli
- Purity: >90.0%
- Predicted molecular weight: 36.8 kDa
- Formulation: lyophilized from 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0
- Storage: store at -20°C; stable for 2 years from receipt
- Available sizes: 20 μg, 50 μg, 100 μg; larger sizes on request
ILEX LIFE SCIENCES LLC Biocolor Sircol™ 2.0 Soluble Collagen Assay (96-well plate format)
The Biocolor Sircol™ 2.0 Soluble Collagen Assay is designed for quantitative measurement of soluble collagen in material from in-vivo and in-vitro sources. The kit employs colorimetric detection in a convenient microplate-based format.This latest addition to the highly cited family of "Sircol™" collagen kits combines an updated microplate format with an improved dye formulation to significantly enhance collagen sensitivity and specificity. This makes it possible to directly analyze collagen from complex samples such as conditioned media during 2D or 3D cell culture.
Apexbio Technology LLC BCA Protein Assay Kit 1000T
BCA fully named Bicinchoninic Acid Assay is a protein quantification method based on the biuret reaction principle Under alkaline conditions protein peptide bonds reduce Cu2 (from copper sulfate) to Cu Subsequently BCA chelates Cu to form a purple-colored complex which strongly absorbs light at a wavelength of 562 nm The intensity of the colored complex is proportional to protein concentration as the reduction of Cu2 or the amount of Cu formed directly reflects the amount of protein present in the solution Thus protein concentration in an unknown sample can be determined by measuring absorbance This assay kit features easy operation high stability high sensitivity and excellent compatibility It can detect protein concentrations as low as 25 g/mL with a minimum detection limit as low as 0 5 g protein It requires a sample volume of only 1 20 L The assay shows good linearity in the concentration range of 50 2000 g/mL
Abcam BCA Protein Assay¿Kit
BCA Protein Assay¿Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam BCA protein assay kit reducing agent compatible (microplate)
BCA protein assay kit reducing agent compatible (microplate)
The product is subject to the following: Abcam Restricted Use Statement